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MCF7 cells transfected with control siRNA (siCTL) or two individual siRNAs specifically targeting HIF1alpha (siHIF1alpha-1 and siHIF1alpha-2) were then cultured in normoxic (20% O2) or hypoxic (1% O2) condition for 6 h, followed by RT-qPCR (E) and IB (F) analysis to examine the expression of PRMT1 and HIF1alpha at mRNA and protein level, respectively (mean ± SEM,**p< 0.01,***p< 0.001).
#Bar Plot#Error Bars#Clustered#MCF7 Cells#siRNA#HIF1alpha#Normoxic Condition#Hypoxic Condition#RT-qPCR#Immunoblotting#PRMT1#mRNA#Protein
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