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Protein lysates of HEK293T cells expressing scr shRNA, EMG1 shRNA, catalytically inactive EMG1 (mut), or wild-type EMG1 overexpression plasmids (O/E) were probed for expression of EMG1 via western blot (n= 3). Shown is densitometry analysis of EMG1 expression relative to GAPDH in varying HEK293T cell lines. Significance was calculated by one-way analysis of variance (ANOVA) (n= 3).

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