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Polysome profiling was performed in TREx-BCBL1-Rta cells depleted of EMG1 or a scr control after 24 h KSHV lytic replication. Total RNA was isolated from traces containing 3 or more polysomes, and expression of the viral genes ORF57 and ORF65 was analyzed via qPCR and normalized to housekeeping genes. Significance was calculated by one-way ANOVA (n= 3).
#Bar Plot#Error Bars#Line Plot#Polysome Profiling#TREx-BCBL1-Rta Cells#EMG1#Scr Control#KSHV Lytic Replication#Total RNA#ORF57#ORF65#qPCR#Housekeeping Genes#One-way ANOVA
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