Western blot analysis of phosphorylated Rb (p-Rb Ser108/111) and total Rb protein in SH-EP cells in response to olaparib and/or palbociclib treatments following control (siCTRL), BRCA2 (siBRCA2), and PALB2 (siPALB2) knockdown. Actin is used as loading control. p-Rb is quantified below each lane normalized to total Rb protein and untreated control (siCTRL).
