Coronal section through the granule cell body layer from contralateral (CONTRA) DG control (upper panels) and ipsilateral (IPSI), HFS-treated DG (bottom panels) at 1 h post-HFS recording. Single-plane confocal images show immunostainings for SRRM2 depicted in green, ARC protein in red, and DNA counterstaining in blue on the merged images. Single nuclei are marked with dashed lines and shown on the right-side panels. The top rows show colocalization color maps of two signals represented in grayscale in the rows below. Regions of high colocalization are shown in warm colors (yellow to red) and regions with negative colocalization are depicted in cold colors (blue to turquoise). Examples of colocalization hotspots are indicated by white arrowheads. Scale bars, 5 μm.
