Histogram showing Piezo1 inward current inhibition from HEK Piezo1delcells transfected with Piezo1 recorded in the inside-out patch configuration at −80 mV in response to a −40 mm Hg pressure stimulation, upon acute application of different fatty acids, including palmitic acid (C16:0), behenic acid (C22:0), oleic acid (C18:1), DHA (C22:6), DHA methyl ester (DHA-ME), and DHA triglyceride (TG) all tested at a concentration of 10 μM. The vehicle ethanol was included in the control solution. Statistical significance (Mann-Whitney U test) is compared with the DHA condition.****p< 0.0001.
