Mean currents elicited by membrane stretch (bottom traces) and recorded in the cell-attached configuration at -80 mV in BMDMs polarized to M2. BMDM M0 cells were first polarized to M2 in complete medium supplemented with IL-4 (20 ng/mL) and IL-13 (20 ng/mL) cocktail for 24 h, and then treated with either EtOH (black traces) or 10 μM 7-KC (in red) for an additional 24 h in LDL-free medium. Left inset: M2 polarization was confirmed byCD206gene expression and is represented as relative expression toTOP1. Right insets: peak current amplitude in EtOH (in black) or 7-KC (in red) condition, comparing WT and KO macrophages.n, number of individual recordings are shown above the traces.N= 2, number of animals from each genotype used for isolation of bone marrow mesenchymal stem cells and differentiation to macrophages. Same mice as donor mice inFigure 1. Data are represented as means ± SEM. A Mann-Whitney U test was used for statistical analysis.∗p< 0.05,∗∗p< 0.01,∗∗∗∗p< 0.0001.
