Raw immunoblot Stx1:CaV2.1 signal ratios from each individual were normalized to the control group mean. Enhanced Stx1:CaV2.1 ratio in immunoprecipitated samples indicates stronger interaction between CaV2.1 and syntaxin 1 in the brains of AD patients (both groupsn= 5). Repeated measures ANOVA withpost hoct test and Sidak correction. Error bars represent ± SEM.*p< 0.05, **p< 0.01 and ***p< 0.001; ns, non-significant.
