Representative images showing hippocampal neurons expressing CRISPR-Cas9 knockout constructs targeting either the 7 subunit of nAChR or firefly luciferase as a negative control. Both constructs also include a GFP marker. Images left to right show different views of labeled neurons incubated with either the fluorescently tagged high-affinity 7-nAChR ligand -bungarotoxin CF594, demonstrating the loss of surface protein in soma, dendrites (D), and axons (A) of cells expressing the 7-nAChR CRISPR construct, or Aos tagged with the fluorophore tetramethyl rhodamine (TAMRA), showing punctate binding to morphologically identified axons (arrowheads) in control cells that is lost in 7-nAChR-knockout axons. Scale bar, 10 m.
