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Quantification ofccl34b.1+microglia (green) located at central OT in uninjured and injured sides of the blank-ctrl (left two panels) orcxcr4b-KO (right two panels) Tg(ccl34b.1:EGFP) zebrafish, which were injured at the early-larval stage (upper panel, 10 dpf–2 dpi; blank-ctrl,n= 14 animals;cxcr4b-KO,n= 10 animals) (I1) or late-larval stage (lower panel, 18 dpf–2 dpi; blank-ctrl,n= 11 animals;cxcr4b-KO,n= 10 animals) (I2) (mean ± SEM; one-way ANOVA, followed by Tukey’s HSD test; ns,p> 0.05,***p< 0.001). In (F) and (H), dashed lines indicate OT boundaries, arrowheads indicate the injury sites. Scale bars, 50 μm. Nuclei are visualized by DAPI (blue). See alsoFigureS8andTableS4.
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