Microscopy from Scientific Research

Representative images (A, H, and N) and cell-number quantification (B, I, and O) of EdU+EGFP+DsRed2+newborn MOL (EdU+, white; EGFP+, green; DsRed2+, red) in the uninjured and injured sides of Tg(olig2:DsRed2;mbp:EGFP) OT at the late-larval (21 dpf-7 dpi), juvenile (55 dpf-7 dpi), and adult (3 mpf-7 dpi) stages, respectively (n= 5 animals at each stage; mean ± SEM; parametric paired t tests; ns,p> 0.05;*p< 0.05;**p< 0.01). In (A), (H), (N), (E), (L), and (R), dashed lines indicate OT boundaries. Boxed areas are shown magnified in the bottom panels. Arrowheads indicate the injury sites. Scale bars, 50 μm (A1 and A2, H1 and H2, N1 and N2, E1 and E2, L1 and L2, R1 and R2) and 5 μm (zoom-in images). Nuclei are visualized by DAPI (blue). See alsoFigures S4andS5.

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