Microscopy from Scientific Research

Immunofluorescence of an iPSC-derived RPE monolayer shows co-staining of all AP-5 components studied here (cyan) with Rab7 (magenta), a marker of late endosomes (left) or TGN46 (magenta), a marker of the trans-Golgi network structures (right side of each panel). White asterisks on higher-magnification images highlight regions of co-localization, revealed either by white areas (indicative of magenta and cyan overlap) or by magenta areas engulfing cyan spots. En-face and cross-sectional views of the same images are shown on the left of each panel. Phalloidin (yellow) stains actin filaments and plasma membranes, and DAPI (blue) stains nuclei. Scale bars, 3 m (images with phalloidin staining) and 1 m (images without phalloidin staining).

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