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Evolutionary relationship of chromosome-resolved Lemnaceae accessions to the angiosperms. The species tree topology was estimated from a concatenated supermatrix alignment (AA) of 854 genes identified as single copy in 87% of the analyzed species, including 11 Lemnaceae clones, 16 other monocots and dicots, andGnetum montanum(not shown) as the gymnosperm outgroup. Blue shading highlights the divergence of the Lemnaceae during the Eocene (Eo).

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