Immunofluorescence of PICH and chromosomes (DAPI) during anaphase. Cells 1 and 2 display PICH-positive ultrafine bridges (UFBs) between two anaphase chromosome masses. Scale bars, 5 m. Graph (right) shows the number of PICH-positive UFBs (length > 2 m) in individual NCAPD2-Cer and NCAPD2-Cer-NLS anaphase cells (n= 69 and 75, respectively) whose spindle length was greater than 8 m. The median and interquartile ranges are shown. Thepvalue obtained using Mann-Whitney U test.
