Mitotic progress of individual NCAPD2-Cer (i), NCAPD2-Cer-NLS (ii), or parental (iii) cells (y axis) plotted against time (x axis) and aligned relative to NEBD (time zero). Cells expressing NCAPD2-Cer and NCAPD2-Cer-NLS were treated with siRNA against endogenous NCAPD2. The colored lines (same color codes as A) refer to trajectories of individual cells through mitosis. The end of the line indicates the end of the time lapse for the cell. The number of analyzed cells for i, ii, and iii were 194, 122, and 99, respectively.