Different strain types heterozygous grown with or without both antibiotics, only apramycin (50 g/mL), or only hygromycin (100 g/mL). Fluorescence microscopy of individual colonies (top rows) and the corresponding quantification of fluorescence (bottom rows) show the expression and colocalization of EGFP (cyan) and mCherry (magenta) in different strains in response to selection. Pixel-wise colocalization of either marker in both channels was quantified as signal intensity for each pixel within the colony area. 10,000 randomly sampled points are plotted. A scatter pattern tending toward either the x (mCherry) or y (EGFP) axis indicates dominance of a single marker, whereas a distribution in the top right region indicates colocalization of cells expressing both markers. Yellow contour lines indicate the density of spots. The bottom-left corner, also showing high pixel density, represents pixels in the black background with no fluorescence.
