Seizure phenotypes were observed when BL exposure occurred at ~24-h intervals throughout development. CS embryos were exposed to BL at 2-h intervals as indicated. Larvae were exposed to BL for 6-h durations (when an effect was not expected) or 2-h durations when an effect was anticipated. CS embryos exposed to BL at 17–19 h AEL exhibited an increased seizure RT (one-way ANOVA,f(10,224) = 19.86,p< 0.0001,n= 15). CS late-stage L1 larvae exposed to BL at 20–22 h ALH (p< 0.0001,n= 15), and L2 larvae exposed to BL for 2 h at 44–46 h ALH (p< 0.0001,n= 15), and 24 h again after that for mid-stage L3 larvae exposed to BL at 68–70 h ALH (p< 0.0001,n= 15); all show an increased seizure RT. The gray data points depict the BL exposure groups that did not have an increased seizure RT at wandering L3 (full descriptive statistics andpvalues are reported inTable S1). Dark-reared controls (“dark”) are shown for comparison.
