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H831 or H052 were preincubated with Hla for 1 h. Thereafter, Hla was washed using centrifugal membrane filters (5 kDa cutoff) and added to cells. Cell viability was measured after 6 h. Viability was restored by QDS upon Hla incubation under (D) but not under (C). Bars represent three experiments with similar results (mean values SD). Statistical significance was determined by unpaired, two-tailed t test compared with inhibitor-free Hla control,n= 3.****p< 0.0001,***p< 0.001,**p< 0.01,*p< 0.05.
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