Microscopy from Scientific Research

Open access visualization of Microscopy, QDS, Hla, Adherens Junctions, Human Lung Endothelial Cells
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QDS prevented Hla-induced degradation of adherens junctions in primary human lung endothelial cells. HPMEC were grown on 8-well coverslips (top left) and incubated with 1 g/mL Hla for 30 min in the absence (top right) or presence of H831 (1.6 M, bottom left) or H052 (1.6 M, bottom right). Cells were fixed in MeOH/acetone (50:50) and immunostained with VE-cadherin antibodies and Alexa647 IgG as secondary antibodies (red). DNA was visualized by DAPI staining (blue). Shown is a representative from three independent experiments with similar results. Scale bar: 20 m. See alsoFigure S1.

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