Schematic diagram showing the lineage tracing strategy for Krt14-positive cells from puberty in the presence of WT or KO of Bcl11b for experiments in j - s . Specifically, Krt14-rtTA/TetO-Cre/mTmG / Bcl11b wt/wt or Krt14-rtTA/TetO-Cre/mTmG / Bcl11b flox/flox mice at 5 weeks of age were pulsed by a high dose of doxycycline (2 mg) and then mated during adult stage for multi-pregnancy. Mammary glands chased for various periods of time indicated during multiple pregnancies (1 st Preg17.5 d, 2 nd Preg17.5 d, 3 rd Preg17.5 d) were harvested for analysis of GFP-labeled cells.
