Microscopy from Scientific Research

Colocalization analysis of GFP-ATG9A and endogenous LC3 on RTN4B-mCherry noncanonical autophagosomes (upper lane) or RTN4B-mCherry vesicles (lower lane) in WT or ATG5 –/– HeLa cells co-transfected with RTN4B-mCherry and GFP-ATG9A. Scale bar, 10 μm. Quantification of colocalization was presented as Pearson’s correlation coefficient ( r ). n = 36, 39 cells from three independent experiments.

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