Microscopy from Scientific Research

Immunofluorescence of RTN4B-mCherry (red) and endogenous LC3 (green) in HeLa cells transfected with RTN4B-mCherry for 36 h. The smaller RTN4B-mCherry puncta with diameters less than 0.2 m are indicated by white arrows, and the larger RTN4B-mCherry puncta with diameters greater than 0.2 m are denoted by blue arrows. Scale bar, 10 m. The data are presented as means SEMs. n = 20 cells from three independent experiments. p values were calculated by Student’s t -test. **** p < 0.0001.

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