Tet-on-GFP-RAB22A Q64L stable HeLa cells were transiently transfected with RTN4B-mCherry for 24 h, followed by the treatment with 50 ng/mL dox for 24 h. Then time-lapse imaging was performed with a Nikon Ti2 spinning disk living cell microscope. RTN4B-mCherry puncta were formed and entered into Rafeesomes. Scale bar, 2 m.
