Immunofluorescence of LC3 in HeLa cells stably expressing GFP-RAB22A Q64L with TMEM33 or RTN4B knockdown. The Ratio of Rafeesomes with a diameter of 1–5 μm and containing endogenous LC3 was calculated relative to total RAB22A-positive vesicles. Scale bar, 10 μm. The data are presented as means ± SEMs. n ≥ 30 cells from three independent experime n ts. p values were calculated by Student’s t -test. **** p < 0.0001.
