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The mixture containing the cytosol of WT HeLa cells and the membrane fractions of ATG5 –/– HeLa-HA-TMEM33 or -RTN4B-FLAG cells was analyzed using western blotting, and the levels of lipidated LC3 (LC3-II) were measured. The data are presented as means ± SEMs. n = 3. p values were calculated by Student’s t -test. ** p < 0.01, *** p < 0.001.

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