The colocalization of endogenous TMEM33 (red) and endogenous LC3 (magenta) with GFP-Vector, GFP-RAB22A WT or GFP-RAB22A Q64L was detected by immunofluorescence. Scale bar, 10 m. Quantification of TMEM33 colocalization with LC3 was presented as Pearson’s correlation coefficient ( r ). The data are presented as means SEMs. n = 40, 41, 47 cells from three independent experiments. p values were calculated by Student’s t -test. **** p < 0.0001. The ratio of Rafeesomes induced by GFP-RAB22A Q64L containing endogenous TMEM33 and LC3 was quantified and calculated relative to total RAB22A-positive vesicles.
