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Schematic workflow of G4switch-mediated optical control of G4-containing gene expression profiled by SLAM-seq. U2OS cells were treated with 9 or DMSO control for 30 min in the dark or under indicated illumination conditions, followed by 4sU labelling over 60 min. RNA was extracted, and the abundance of newly synthesized RNAs was quantified by analysing T > C conversions through the SLAM-seq protocol.

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