In the two-cutoff approach, the cutoffs for Lumipulse p-tau217 were <0.22 pg ml -1 and >0.34 pg ml -1 , <1.59 pg ml -1 and >2.92 pg ml -1 for MS-based p-tau217 and <3.55 pg ml -1 and >5.08 pg ml -1 for MS-based %p-tau217. Data are presented as the observed percentage and the error bars as the 95% CI derived from the bootstrap distribution. AD pathology was defined as CSF A42:p-tau181 < 11.94 or positive visual read on amyloid PET if lumbar puncture was not performed. To assess whether the observed difference in the statistics is significantly different from zero, we performed a bootstrap hypothesis test. The P value (two sided) was calculated as the proportion of bootstrap resamples ( n = 2,000) where the absolute null-distributed statistic was greater than or equal to the observed difference. Differences between AUCs were assessed using DeLong statistics. Results were not corrected for multiple comparisons. Significant P values in the order as presented in the plot: 0.020
