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Pathway of starch synthesis in wheat endosperm. Gene expression dynamics of enzymes and transporters from sucrose import to starch synthesis in wheat endosperm 45 . Metabolites are in gray letters, enzymes in black. log 2 FC of gene expression from four biological replicates per condition at 4 h (left-hand column of squares) and at 24 h (right-hand column of squares) comparing DMNB-T6P treatment with control. Values of gene expression are colored from blue to red. All log 2 FC values are capped to 2. Dashed lines indicate multiple steps. AGPL/S, ADP-glucose pyrophosphorylase large/small subunit; BGC1, B-granule content 1; Bt, ADP-glucose brittle-1 transporter; cPGM, cytoplastic phosphoglucomutase; DPE, disproportionating enzyme; FRK, fructokinase; GBSS, granule bound starch synthase; ISA, isoamylase; PFK, phosphofructokinase; PGI, glucose-6 phosphate isomerase; PHS1, plastidial -glucan phosphorylase; PTST1, protein targeting to starch; PUL, pullulanase; SBE, starch branching enzyme; SS, soluble starch synthase; SUS, sucrose synthase 3; SUT, sucrose transporter; UGP, UDP-glucose pyrophosphorylase. -Glc, glucose; Frt, fructose ( n = 4).

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