Mutation-Simulator 63 was used to add random single nucleotide base substitutions to 676 EBOV RdRP sequences obtained by Seq-Well sequencing 39 at increasing mutation rates. We performed ten simulations per mutation rate. The sequences were subsequently aligned using kallisto translated search against the complete PalmDB, Kraken2 translated search against the RdRP amino acid sequence of EBOV with a manually adjusted NCBI Taxonomy ID (to allow compatibility with Kraken2) and kallisto standard workflow against the complete EBOV nucleotide genome ( GCA_000848505.1 ). The plot shows the recall percentage of the 676 sequences for each of the ten simulations at each mutation rate. Each was fitted with an inverse sigmoid for mutation rates of >0.
