Stacked Bar Plot from Scientific Research

Precursors quantified by CHIMERYS, DIA-NN and Spectronaut in at least one (orange) or two (gray) out of three replicate 2-h DIA single-shot measurements of two conditions ( n = 6). Data taken from the LFQbench-type dataset and acquired on an Orbitrap QE HF-X with 8-Th isolation windows. Identifications are filtered at 1% run-specific precursor-level FDR or additionally at 1% run-specific precursor-level eFDR ( Supplementary Methods ).

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