I Cs + densities obtained from voltage ramp recordings shown in ( c ) at either 22 °C or 38 °C were independent of the genotype. Thus, TRPV4 is unlikely to affect murine CatSper temperature activation. Specifically, at - 80 mV, current densities (pA/pF; n = 6) were: - 92.05 ± 9.9 (at 24 °C) and - 239.95 ± 18.8 (at 38 °C) for TRPV4 -/- sperm cells. I Cs + densities (pA/pF; n = 7) at - 80 mV for wild-type sperm cells were - 58.05 ± 5.8 (at 24°C) and - 183.07 ± 30.8 (at 38 °C). Similarly, I Cs + densities at + 80 mV (pA/pF; n = 6) for TRPV4 -/- cells were 147.84 ± 7.3 (at 24 °C) and 254.13 ± 14.1 (at 38 °C). For wild-type cells at + 80 mV, I Cs + densities (pA/pF; n = 7) were 175.27 ± 12.4 (at 24 °C) and 298.77 ± 17.8 (at 38 °C). Data are mean values ± S.E.M. Insert: Mouse genotyping is shown.
