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Time course of PWD/PhJ BMDMs cell death quantified via Propidium Iodide uptake. The graph shows the median value ± SEM, N = 4. Cells were pre-stimulated for 24 h with IFNγ (continuous line) or left untreated as control (dashed line) and then infected with VAND ∆UPRT, ∆GRA12, ∆GRA12::GRA12-HA or left uninfected. The number of dead cells is expressed as a percentage of the total at each time point.
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