Scatter Plot from Scientific Research

Scheme of the CRISPR screen of type I RH secretome in PWD/PhJ BMDMs to identify Toxoplasma factors important to survive IFNg-mediated restriction. The KO pool was used to infect BMDMs either untreated or pretreated with IFNg for two consecutive lytic cycles. Surviving parasites were expanded in fibroblasts prior to gDNA extraction and sgRNA amplification for sequencing. The relative abundance of each guide (L2FC) in IFNg-restricted versus untreated parasites indicates the importance for in vitro survival of the relative Toxoplasma gene.

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