Bacterial loads in organs of C3HeB/FeJ mice infected intravenously (10 6 CFU/animal) with R, RΔ 4690c and MAB_4690c :: 4690c . Mice were sacrificed at days 1, 7, 15, and 30. Lungs ( c ), kidneys ( d ), liver ( e ), and spleen ( f ) were collected, diluted, and cultured on VCA3 agar plates (n = 5). CFU were counted after 5 days. Results are expressed as median with interquartile range. Each dot in the scatter plots represents an individual mice. At 15 dpi, p = 0.0216 (RΔ 4690c vs. RΔ 4690 c ::4690c ) in lungs; at 15 dpi, p = 0.0314 (R vs. RΔ 4690c::4690c ) and at 30 dpi, p = 0.0074 (R vs. RΔ 4690c ) in kidneys; p = 0.0067 (R vs. RΔ 4690c ) at 30 dpi in liver, p = 0.0139 (R vs. RΔ 4690c ) and p = 0.0397 (RΔ 4690c vs. RΔ 4690 c ::4690c ) at 30 dpi in spleen. P values were determined using the log-rank (Mantel-Cox) test for Kaplan-Meier survival curves to assess survival statistics significance ( a , b ) and by two-tailed unpaired t- test ( c – f ) using GraphPad prism program; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Source Data are provided as a Source Data file.
