The phylogenetic tree was inferred from GBDP distances calculated from complete genomic sequences of 42 mycobacterial strains. For the 1st GPL locus, bioinformatic analysis of NRPSs shows that the deduced product is ubiquitous and identical in all GPL-producing mycobacteria (green squares). However, not all GPL-producing mycobacteria systematically have a second locus. For this one, the nature of the deduced product differs, as illustrated by the different colored squares. Note that this second locus is conserved in the M. abscessus complex and in only closely related phylogenetic strains (yellow squares). Color code used: For the Species column, each species is differentiated by a different color. For the locus columns, white for absence or black for presence. For the Product columns, each color represents a different predicted structure (Data S1 ).
