Distribution of Caco-2-mScarlet (red) cells in different types of tumors. (left) In the flat monolayer tumor foci, Caco-2 cells (T) were present directly adjacent to the normal epithelial cells (N) that were negative for mScarlet. Caco-2 cells also formed ACF-like foci, which were exclusively composed of mScarlet-positive tumor cells (center), and protruded, pedunculated tumors supported internally by host stromal cells (right). These tumors (T) were also found in proximity to the normal epithelium (N)