Expression of MMP2/MMP9 at the gap edge in the in vitro wound healing model. After removal of the insert HaCaT cells were incubated in absence of BK (top panel), with 500 nM BK (middle panel) or cBK (bottom panel) for 24 h. Cells were then immunostained against MMP2 (green) and MMP9 (red). DAPI (blue) was used as a nuclear counterstain. Wound edge has been additionally marked with a dotted line (white). Representative images. Scalebar 20 m