Expression of B 2 /B 1 receptors at the gap edge in the in vitro wound healing model. After removal of the insert HaCaT cells were incubated without (top panel), with 500 nM BK (middle panel) or cBK (bottom panel) for 24 h. Cells were then immunostained against B 2 (green) and B 1 (red) receptor. DAPI (blue) was used as a nuclear counterstain. Wound edge has been additionally marked with a dotted line (white). Representative images. Scalebar 20 m
