EMSA experiments were performed testing the binding of human SMAD1/3-MH1 domains to differently oriented pGC-SBE/SBE heterocomposite motifs. Protein concentrations (M) are shown on top of the EMSA. Abbreviations for the DNA oligonucleotides and dsDNA sequence are shown above. Single, double, and triple SMAD-MH1 binding to dsDNA is indicated with black triangles.
