Bar Plot from Scientific Research

Schematic of the Car5b region indicating the location of B6-Xi primer pairs. B6-R for P4 and B6-R2 for P2 are the same primers shown in Additional file 1 : Fig. S7A. Additional B6-Xi primer (B6-r1 for P1) were designed using validated SNPs. For each region, the two closest DpnII sites (D) upstream of each primer are marked together with the distance between the primer and the closest DpnII site and between two DpnII sites. DpnII sites are numbered D1-D7. Note that due to the inversion of P4, D7 rather than D5 is the second closest site upstream of B6-R in Xi-Inv. The deleted or inverted P4 region (1864 bp) is indicated as a gray box.

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