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13 samples were captured over 4 10x Genomics Visium OCT slides and 3 FFPE slides, and sequenced over 5 runs on an Illumina NextSeq 2000. Samples are categorized by sex, genotype, tissue preparation protocol, library construction protocol, and tissue placement. A matching scRNA-seq sample of 3 mouse spleens was captured over 1 gel bead-in emulsion (GEM) well, and gene expression and hashtag oligos (HTOs) were sequenced over 1 Illumina run. Subsequent data analysis involved processing with 10x Genomics Space Ranger 2.0.0, and quality control, feature selection, dimensionality reduction, and downstream analysis using various R-based software packages. Figure created with BioRender.com .

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