Gel from Scientific Research

BIK1 phosphorylates DGK5b, but not DGK5a in vitro. The in vitro kinase assay was performed using purified GST-BIK1 or GST-BIK1 KM as a kinase and GST, GST-BAK1 KD , GST-DGK5b, or GST-DGK5a as the substrates using [g- 32 P]-ATP. BIK1 KM is a BIK1 kinase-dead mutant, and BAK1 KD is the BAK1 truncation with the kinase domain alone, which does not carry auto-phosphorylation activity. Phosphorylation was analyzed by autoradiography (Autorad.) (top), and protein loading is shown by CBB staining (bottom).

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