Gel from Scientific Research

DGK5a exhibits a compromised diacylglycerol kinase activity. The recombinant GST-MBP, HIS-DGK5b, or HIS-DGK5a proteins were incubated with [ 14 C]-DOG in a reaction buffer containing ATP for 30 min. Chloroform-soluble products were separated by the thin-layer chromatography (TLC) plate, and PA was detected by autoradiography (Autorad., top). The amount of PA levels is quantified based on the band intensities using ImageJ software. The PA level produced by DGK5b (second lane) was set as 1, and relative PA levels were labeled on the bottom of the autoradiography panel. The protein loading is shown by CBB staining on the bottom.

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