Gel from Scientific Research

Immunoprecipitation of endogenous RNA Pol II using the ESC line described in A whereby Polr2a was targeted with a knock-in SPOT epitope tag. The immunoprecipitation was performed with magnetic beads coated with anti-SPOT antibodies. The efficiency of RNA Pol II IP and RNA Pol II O-GlcNAc levels were probed by WB analysis at different time points after Dox-induction of BtGH84-NLS expression.

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