Gel from Scientific Research
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Protoplast transient assay system to examine the competition between AtMTA and AtALKBH10B for binding to Ser5P-CTD of RNA Pol II. Protoplast cells obtained from alkbh10b-2 mutant and WT plants were used for 35S:AtMTA-GFP transformation. Total proteins extracted from protoplast cells were immunoprecipitated with anti-Ser5P-CTD and analyzed by western blot with antibody to GFP
#Gel#Protoplast Transient Assay#Competition Assay#Protein Binding#AtMTA#AtALKBH10B#Ser5P-CTD#RNA Pol II#alkbh10b-2 Mutant#WT Plants#35S:AtMTA-GFP#Immunoprecipitation#Western Blot#Antibody to GFP
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