Illustration from Scientific Research

Open access visualization of Illustration, Next Generation Sequencing, Amplicon Sequencing, Genome, Off-Target Sites
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Next generation amplicon sequencing analysis of the top 9 out of 10 predicted off target sites in the genome showed no detectable edits above the 1% threshold within the editing window and surrounding bases. Mismatch bases between the sgRNA and potential off-target binding sites are highlighted in different colours.

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