Illustration from Scientific Research

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Next generation amplicon sequencing analysis of the top 9 out of 10 predicted off target sites in the genome showed no detectable edits above the 1% threshold within the editing window and surrounding bases. Mismatch bases between the sgRNA and potential off-target binding sites are highlighted in different colours.
#Illustration#Next Generation Sequencing#Amplicon Sequencing#Genome#Off-Target Sites#sgRNA#Binding Sites
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