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Quantitative analysis of the cell viability percentage and Ki67 positive rate of HCOs using ImageJ (n= 3). All data were presented as means ± SD. Comparisons between two groups were performed using two-tailed unpaired Student's t test, and statistical significance was indicated by asterisks (*) above the lines. NS indicatesp> 0.1234. *p< 0.0332, and **p< 0.0021. ***p< 0.0002, and ****p< 0.0001.

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