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Scanning fluorescence microscope with photon-counting detection (APD) of fluorescence passing the dichroic mirror (DM) and a confocal pinhole (PH). The interference of two beams with adjustable phase difference entering the pupil of the objective lens creates an illumination intensity pattern in the focal plane featuring x orientated or y oriented line-shaped diffraction minima and maxima (MINFLUX set-up). Two fluorophores are sketched as stars.

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