Initial target selection in HeLa cells expressing mRFP-GFP-LC3 using fluorescence microscopy (colors) overlaid on reflected light (grayscale). Green and yellow: autophagosomes; red: autophagolysosomes. The light microscope field of view (FOV) is denoted by the white rectangle in the top left inset, showing the grid square in the SEM overview image. Scale bar: 5 μm and 50 μm (inset)
